CELL DISRUPTION
PHYSICO-MECHANICAL METHODS
LIQUID SHEAR
SLURRY PASSES THROUGH NO RETURN VALVES
LARGE SCALE ENZYME PURIFICATIOM
HIGH PRESSURE HOMOGENIZERS EFFECTIVE FOR MICROBIAL DISRUPTION
IMPINGES AGAINST OPERATIVE PRESSURE
WORKS ON PRINCIPLE OF PRESSURE DROP ACROSS VALVES
SOLID SHEAR
PRESSURE EXTRUSION THROUGH ORIFICE AT -25 C
IDEAL FOR TEMPRATURE LABILE
DISRUPTION DUE TO ICE CRYSTALS
AGITATION WITH ABRASIVES
ROTATING DISCS AND SMALL BEADS IN A DISINTEGRATOR
BEADS- GLASS, ALUMINA CERAMICS AND TITANIUM
DISSIPATION OVERCOME BY COOLING JACKET
FREEZE THAWING
OF MICROBIAL CELL PASTE- ICE CRYSTAL FORMATION
THAWING CAUSES DISRUPTION
SLOW , LIMITED RELEASE OF CELLULAR MATERIALS
ULTRASONICATION
20 kHz VIBRATION LEADING TO CAVITATION AND SHOCK WAVES
PROBES
SHORT WORK LIFE
SHORT RANGE
VOLUMES UPTO 10 CM3 ARE EFFECTIVE
CHEMICAL METHODS
DETERGENTS AND ALKALI TREATMENTS
DETERGENTS
DAMAGE LIPOPROTEIN
COMPOUNDS
QUART AMMONIUM
SDS
SODIUM LAURYL SULPHATE
TRITON X-100
PROTEIN DENATURATION
ALKALI
HYDROLYSIS OF CELL WALL
ENZYME
pH- 11.5-12.5
20-30 MINS
OSMOTIC SHOCK
CAUSES SUDDEN CHANGE IN SALT CONC.
EFFECT IS MINIMAL
ENZYME TREATMENT
HYDROLYZE SPECIFIC BONDS BY ENZYMES
STREPTOMYCES
LEUCOCYTES
MICROMONOSPORA
PENICILLIUM
TRICODERMA
EXPENSIVE AND PRESENCE COMPLICATE DOWNSTREAM
USED FOR [RETREATMENT