Biology 311C

Unit 1

Bonds

Intramolecular

Covalent

Nonpolar

Polar

Ionic

Intermolecular

Dipole-Dipole

Hydrogen Bond

Ion-Dipole

Hydrophobic

Van der Waals

Organic Compounds

Isomers

Structural

Geometric

Enantiomers

Carbon skeletons

Length

Branching

Double Bonds

Rings

Functional Groups

Hydroxyl (-OH)

Carbonyl (-C=O)

Carboxyl (-COOH)

Amino (-NH3)

Sulfhydryl (-SH)

Phosphate ((-PO4)^3-)

Methyl (-CH3)

Unit 2

DNA Replication

Experiments

Griffith and Avery, MacLeod, and McCarty

With S - Mouse Dies

With R - Mouse Lives

With Heated S - Mouse Lives

With Heated S + R - Mouse Dies

Messleson and Stahl

Semiconservative (Proven)

Conservative

Dispersive

Hershey and Chase

35S-Labeled Protein

32P-Labeled DNA in pellet

Chargaff's Rule

Process

Parts

Origin of Replication (ORI)

Leading Strands

Lagging Strands

Okazaki Fragments

Replication Forks

Proteins

Helicase

SSB

Topoisomerase

Primase

DNA Polymerase III

DNA Polymerase II

DNA Ligase

Gene Expression

Transcription

Process

Parts

Promoter

Transcription Start Site (+1)

Template Strand

Terminator

Stages

Initiation

Elongation

Termination

Proteins

Prokaryotes

RNA Polymerase

Eukaryotes

RNA Polymerases I,II, & III

Poly-A tail

5' cap

Translation

Process

Proteins

Ribosomes

Amino acids

Amino acyl tRNA synthetase

Peptidyl transferase

Initiation Factors

Elongation Factors

Release Factors

Parts

mRNA

Codons

Anticodons

tRNA

Subtopic

Peptide Bonds

RNA Processing

Parts

Introns (Removed)

Exons (Kept)

Spliceosomes

Unit 3

Energy

Metabolism

Catabolism

Anabolism

Thermodynamics

1st Law

2nd Law

Forms of Energy

Free Energy

DG<0 - spontaneous

DG=0 - equilibrium

DG>0 - nonspontaneous

DG = DH-TDS

ATP

Work Done by ATP

Chemical

Transport

Mechanical

Energy Coupling

Endergonic + Exergonic; DG<0

Energy Flow

Energy Flows through ecosystems; energy lost through heat

Matter cycles within ecosystem

Cell Membrane

Components

Phospholipids

More Unsaturated Lipids - Fluid

More Saturated Lipids - Viscous

Proteins

Functions

Transport

Enzymes

Signal Transduction

Cell-Cell Recognition

Intercellular Joining

Cytoskeleton and ECM attachment

Types

Integral

Peripheral

Cholesterol

Maintains Fluidity

Permeability

Transport

Active

Molecules going against
or up concentration gradient

Requires Energy (ATP)

Proton Pumps
Pump out H+

Ion Pumps
Pump out K+ and Na+

Simple Diffusion

Small, nonpolar molecules

Osmosis

Tonicity

Hypotonic
Less solute

Isotonic
Same cencentration of solute

Hypertonic
More solute

Facilitated Diffusion

Large and/or Polar molecules

Channel Proteins

Carrier Proteins

Action Potential

Resting State

Depolarization
Na+ gate opens

Rising phase
More Na+ gates open

Falling phase
K+ gates open
Na+ gates close

Undershoot
Cell slightly more negative
than resting state

Endocytosis and
Exocytosis

Phagocytosis

Pinocytosis

Receptor-Mediated Endocytosis