a Puteri Nur' Ain 8 éve
396
BIOPROCESS
Aflatoxin M1 contamination in dairy products, such as milk, cheese, and yogurt, varies based on several factors including the type of product, manufacturing processes, and seasonal conditions.
Megnyitás
CONTAMINATION OF DAIRY PRODUCTS BY AFLATOXIN M1 AND ITS CONTROL BY PROBIOTICS CONCLUSION AND RECOMMENDATION Apply ideal recommend limit to minimize the AFM1 contamination in milk Widespread and continuous training for both the producers and consumers Maintain low levels of AFB1 in the feeds of dairy animals Milk and dairy product have to be controlled continuosly RESULT Concentration of AFM1 in yoghurt depends on some variable country and season condition of yoghurt ripening type of yogurt manufacturing procedures Concentration of AFM1 in cheese depends on some variable production technologies water content type of cheese The results obtain depends on the country Aflatoxin mostly found in raw milk samples Mozarella cheese have the highest rate exceeding the permissible rate 1 out of 9 isolates of A.flavus produced aflatoxin Probiotics use Play important role in digestive and respiratory Bifidobacterium lactis more ability to remove AFM1 involves physical binding of the toxin to the bacterial cell wall AFM1 bind to milk protein Example Bifidobacterium Lactobacillus reducing the availability of AFs METHOD Detoxification of AFM1 in Yoghurt by Lactic acid Bacteria Binding ability of LAB in AFM1 contaminated milk Preparation of lactic acid bacteria inoculum Cultures activation ELISA TEST Statistical Analysis AFM1 evaluation Sample preparations AFM1 determination Determination of toxigenic of A.flavus Isolation and identification of moulds Preparation of sample AFLATOXIN M1 Possible accumulation and linkage to DNA risk more to the infants Relatively stable during pasteurization and storage Not destroyed Found when lactating animals are fed with contaminated feedstuff The hydroxylated matabolite of aflatoxin B1 Less carcinogenic and mutagenic than AFB1 AFLATOXIN It also can cause liver damage and cancer Contamination occur from storage handling harvest field It can grow under favourable temperature and humidity Highly toxic produced by the species of Aspergillus A. parasiticus A. flavus
