Image acquition

detector

photomultiplier

hybrid

3D imaging

slower

pinhole

multiple pinholes

photobleaching

reduced

sensitive

more sensitive

specimen

bigger, live imaging

pinhole

multiple

scanning speed

very fast

Subtopic

detector

array based detector

2D/3D imaging

rapid

pinhole

diameter can be changed

photobleaching

higher

sensitivity

less sensitive

specimen

smaller specimen, non-live imaging, living cells

pinhole

single

bigger

light sensitive

for optical scetioning

scanning speed

slow

better signal/noise ratio