SITOGENETIKA

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TEKNIK PENGAMBILAN DATA

door Ayu Trisnasari

Metode pengambilan data

door 097_Ni Nyoman Ratih Wijayanti

LIMS ATMOS

door Tiga Pinguin

kerajinan tekstil

door Felia T.P.H.P

SITOGENETIKA

Type in your name

FISH (Fluoresence In Situ Hybridization)

Tujuan

Untuk menunjukkan suatu sekuens DNA (area of interest) secara langsung (in situ) pada preparasi kromosom

Prinsip

Visualisasi dengan fluorescein labelled avidin

Hibridisasi DNA probe berlabel biotin yang berkomplementer dengan DNA target (kromosom)

Denaturasi DNA kromosom dan probe

Sel dalam keadaan metafase/interfase yang terfiksasi pada object glass

Pemeriksaan

Personal skills

Skills can be truly valuable when it comes to convincing a potential employer. Along with work experience and education, your skills will help the employer get a better understanding of your interests and values.

Indikasi

Pasangan

riwayat tidak subur

keguguran spontan berulang

Ibu hamil

riwayat pernah melahirkan bayi cacat

usia > 35 tahun

Individu

keterbelakangan mental

ingin operasi ganti kelamin

kecenderungan bertindak kriminal

jenis kelamin yang tidak jelas

cacat bawaan

Kegagalan tanda-tanda kedewasaan pada pria dan wanita

Amenorhea primer

Wanita dengan postur tubuh pendek (<140 cm)

Orang tua dari anak dengan kelainan kromosom

Kecurigaan adanya kelainan kromosom klasik

Sampling

Amniocentesis

Chordocentesis (darah dari umbilical cord)

CVS (Chorion Villus Sampling)

Punksi sumsum tulang belakang

Darah perifer

Teknik analisis

karyotyping

Jumlahin kromosom

jika ada kelainan 2 kromosom (nomor terkecil)

46, XY, dup (13)(q14)

46, XX,del (1)(q21)

koma lagi jika ada kelainan

47, XXY

47,XY,+21

Koma dengan kromosom seks

ISCN

Menyusun kromosom

klasifikasi Denver

pengecatan

Specify any other skills which you believe qualify you for the position you are applying.

Giemsa

preparasi slide

These skills enhance your interactions, job performance and career prospects. Soft skills are broadly applicable both in and outside the workplace, and relate to a person's ability to interact effectively with coworkers and customers.

G-banding

dibuat preparat

Harvesting

Computer skills you master


Companies are becoming more and more dependent on technology, so your computer skills can easily increase your chances of getting a job that requires such skills. Specify the types of computer skills you master.

Fiksatif

What software do you use for graphic design?

Acetic acid

Carnoys/methanol

mengelurakan air dari sel

tingkatin untuk mengikat warna cat

permberian larutan hipotonik

What operating systems are you familiar with?

KCl

stop pembelahan sel

What kind of computer applications do you use?

kolkisin

Kultur darah

Your language skills can play an increasingly important role in the workplace.

Specify what languages you know

taking into consideration the levels of understanding, speaking and writing.

diteteskan medium

Specify what languages you know

taking into consideration the levels of understanding, speaking and writing.

RPMI

Antibiotik

mencegah kontaminasi

PHA

menstimulasi mitosis

FCS/PBS

Level of understanding


What is your level of understanding?

meningkatkan pertumbuhan sel

pengambil darah

tabung heparin

Level of writing


What is your level of writing?

sel darah putih

Level of speaking


What is your level of speaking?

keadaan steril

Level of understanding


What is your level of understanding?

Pengertian

Pemeriksaan kromosom yg dpt diperiksa pd mikroskop cahaya.

Kromosom

Work experience

This is one of the most important sections in your curriculum vitae. Make sure you specify all your previous work experience, part-time jobs, vacation jobs, voluntary work, and unpaid work experience that are relevant for the position you are applying for.

kelainan

Numerikal

Polyploidy

dst

heksaploid (6n)

pentaploid (5n)

tetraploid (4n)

Triploid (3n)

Monosomy

kekurangan 1 kromosom

Trisomy

3 kromosom

Struktural

Isochromosome (i)

Ring (r)

Inversi (inv)

Derivasi (der)

Duplikasi (dup)

Delesi (del)

Translokasi (t)

Penggolongan (Denver)

G

2 pasang terkecil, akrosentrik, bersatelit

22

21

F

2 pasang metasentrik kecil

20

19

E

submetasentrik kecil

18

17

metasentrik medium

16

D

akrosentrik besar & bersatelit

15

14

13

C

x

6-12

metasentrik & submetasentrik

wanita

8 pasang

laki-laki

7 pasang

B

2 pasang submetasentrik

5

A

1 pasang submetasentrik terbesar

2

2 Pasang metasentrik

3

1

Bentuk

Tipe

Gonosom

kelamin

23: XX atau XY

Autosom

Tubuh

kromosom 1-22

Subtopic

Letak sentomer

Telosentrik

paling ujung

Akrosentrik

hampi dekat ujung

Submetasentrik

dekat lengan P

antara tengah & ujung

Metasentrik

lengan p=q

di tengah

Stuktur

Struktur

Sentromer

lekukan

kinetokor

perlekatan benang spindel

lengan

panjang (q)

pendek (P)

kromomer

satelit

telomer

ujung

Identifikasi

Specify your work experience

Start with the most recent period.
Example: Jul 2013 - Jun 2016.

2 kromatid

diikat sentromer

Membelah

replikasi

kromatid

Interfase

1 kromatin (di inti)

cara pemberian nama/istilah

ISCN (International System for Human Cytogenetic Nomenclature)

Jumlah

What were/are your responsibilities in this position?

46, XY

46, XX

diamati pd tahap metafase

What industry does the company or institution belong to?

di inti sel

Name of the company or institution where you last worked or where you are currently working.