Identify regions of the Y chromosome in the reference assembly that include genes
Validate that they are really on the Y
Determine the tissue and time of gene expression
Isolate RNA from different developmental stages and tissues
Determine the relative expression of target genes through qPCR
What kind of genes exist on the Y?
Where can we insert marker genes on the Y for population control purposes? (Sterile Insect Technique) We need a region where the marker will be stably expressed and easily integrated
Characterize the function of Y genes
Assess the gene expression reduction levels through qPCR
Activate RNAi through injection of dsRNA on the appropriate developmental stage of the insect
Characterize the phenotype caused by the reduction of gene expression
PCR on the region of interest with male and female DNA
RACE PCR and sequencing to assess the complete transcript sequence
