glycoproteins
glycolipids
an example of potential energy
deoxyribose has one less hydroxyl group than ribose

chemical bonds, cell structure and function

membrane transport ^

membranes are found around the cell and each organelle

The cell boundary

seperates cellular materials from external environment

regulates which materials can enter and exit the cell

maintains homeostasis in cell

Semipermeable membranes

membranes that allow certain materials to pass through based on certain properties

size, hydrophobicity, charge

The fluid mosaic model

the membrane is made up of many smaller parts and the structure moves likes a fluid

Phospholipids and proteins

make up most of the membrane

cholesterol helps with flexibility and carbohydrate chains help communicate with other cells

phospholipid: 2 fatty acid tails and a phosphate head

phospholipid bilayer forms because the inside and outside of the cell are mostly water

semipermeable because it only allows certain molecules to cross

Hydrophilic, polar, large and charged molecules must use a transport Protein to enter/exit the cell.

The proteins are specific; glucose can only pass through a glucose transport protein.

transport proteins

There are two types of Transport Proteins: Protein Channels: a special entryway for large, polar, hydrophilic and charged ions to diffuse through the cell membrane This is called Facilitated Diffusion.

cells

chemical evolution hypothesis

three domains of life

bacteria

c

no nucleus

DNA in nucleoid

no membrane bound organelles

membranes

plasma membrane

consists of phospholipid bilayer that is semipermeable

hydrophobic fatty acid tail (away from water) and a hydrophilic head (faces water)

amphipathic

cholesterol

hydrophilic becuase of phophate group

regulate cell's tarffic

membrane fluidity

temp affects the fluidity

above temp lipid is in liquid crystalline phase and is fluid

below temp lipid is in gel phase and is rigid

Each phospholipid has a specific temp

membrane proteins

functions

transport

passive transoport

diffusion of a substance across a membrane with no energy investment

example includes osmosis

water balance of cells

tonicity

ability of a surrounding solution to cause a cell to gain or lose water

isotonic

solute concentration is the same as inside the cell; no net water movement across the plasma membrane

hypertonic

solute concentration is greater than that inside the cell; cell loses water

hypotonic

solute concentration is less than that inside the cell; cell gains water

facilitated diffusion

passive transport aided by proteins

active transport

movement of substances from low to high concentrations

maintains a concentration gradient

uses energy

specific case of active transport is the sodium-potassium pump

enzymatic actvity

signal transduction

cell-cell recognition

intercellular joining

attachment to ECM an cytoskeleton

eukaria

made up of eukaryotes

cells have membrane bound nucleus

most of the DNA is in the nucleus

nucleus is an organelle that is bounded by a double membrane

archaea

branched membrane lipids

extreme halophiles: live in saline environments

extreme thermophiles: very hot environments

methanogens live in swamps and produce methane as a waste product

strict anaerobes

biological macromolecules

as

Proteins

protein polymers

bonded through peptide bonds

has directionality (N- terminal & C-terminal ends)

Structure and Organization

Primary: types, quantity, and sequence of amino acids

Secondary: formation of α helices or β pleated sheets

occurs due to the formation of hydrogen bonds

Tertiary: 3D-shape of polypeptide chain

determined by R group interactions

Quartenary: arrangement of multiple polypetide chains to form a protein

monomers: amino acids

r

20 different amino acids are used by living organisms

Nucleic Acids

bonded through phosphodiester bonds

is formed through dehydration synthesis

also known as condensation reaction

results in the sugar-phopshate backbone

has directionality (5' & 3' ends)

polymers:

RNA

base pairs:

adenine (A)

uracil (U)

cytosine (C)

guanine (G)

sugar molecule: ribose

forms a single-stranded nucleotide chain

DNA

base pairs:

adenine (A)

thymine (T)

cytosine (C)

guanine (G)

sugar molecule: deoxyribose

forms a double helix
with anti-parallel strands

connected by base-pair hydrogen bonding

monomer: nucleotide

nitrogenous bases

pyrimidines

cytosine

thymine

uracil

purines

adenine

guanine

sugar molecule

phosphate group

Carbohydrates

carbon-based molecules hydrated with many hydroxyl groups (-OH)

monomers: monosaccharides

polymers: polysaccharides

bonded through glycosidic linkages

form via a dehydration reaction

Add your text

types:

simple carbohydrates

most abudant is glucose

C6H12O6

complex carbohydrates

cellulose

composed of beta glucose molecules

cannot be digest by humans because we
lack the necessary enzyme needed to break it down

starch

composed of alpha glucose molecules

can be digest by humans due to the
amylase enzyme

Lipids

r

not a true polymer

fatty acids

saturated

saturated with hydrogen

single bonds

able to pack closely together

higher melting points

solid at room temperatue

unsaturated

not fully saturated with hydrogens

double bonds

causes kinks in the chain

liquid at room temperature

phospholipids

contains a phosphate group

otherwise known as the "head"

hydrophilic

composes all cell membranes

has two fatty acid chains

otherwise known as the "tail"

hydrophobic

triglycerides

a lipid with 3 fatty acid chains

linked to a glycerol molecule

occurs through a dehydration reaction

steroids

made up of 4 fused carbon ring structures

cholesterol

essential for the structure of
animal cell membranes

waxes

functions:

protection

prevention of water loss

fatty acids bound to long chain alcohol molecules

Chemical bonds

Covalent bonds

strongest bond: sharing of electron pairs

two types:

polar covalent

unevenly matched, but willing to share

example of covalent bond: two hydrogen bonds getting close together, the attraction is balanced in both directions. Hydrogen gas is formed.

nonpolar covalent

evenly matched

Add your text

Ionic bonds

attraction between ions of opposite charges

complete transfer of valence electrons between atoms

metal loses electrons to become a positively charged cation

non metal accepts these electrons to become a negatively charged anion

example of ionic bond: Na and Cl

Ion- dipole

attractive forces between polar molecules and ions

Hydrogen bonds

attractive force between the hydrogen attached to an electronegative atom of one molecule and one from a different molecule

the electronegative atom is usually oxygen, nitrogen, or fluorine.

these have partial negative charges

example of hydrogen bond: a hydrogen atom covalently bonded to an oxygen via a shared pair of electrons

Metallic bonds

type of bonding found in metallic elements

electrostatic force of attraction between positively charged ions and delocalized outer electrons

refers to an interaction between delocalized electrons and the metal nuclei

Example of metallic bonding: if metal cations and electrons are oppositely charged they will be attracted to each other and also other metal cations

Peptide bond

proteins are linear polymers composed of amino acids linked by a peptide bond

chains containing less than 50 amino acids are peptides

chains containing greater than 50 amino acids are called proteins

peptide bonds are formed by the condensation of the carboxyl group of amino acid and the amino group of the second amino acid with the elimination of water

Phosphodiester bond

make up backbone strands of DNA and RNA

this bond is the linkage between the 3" carbon atom of one sugar molecule and the 5" carbon atom of another

these bonds are central to all life on earth

Glycosidic bond

type of covalent bond that joins a sugar molecule to another group which could be another carbohydrate.

biological importance

covalent

holds together the long chains of macromolecules (DNA, RNA, and Proteins)

ionic

compounds with ionic bonds split into ions in water. Ions conduct electricity. Gives specialized cells excitable properties

hydrogen

makes water molecules stick together. responsible of the properties of water.

cause protein chains to spiral and bend, giving unique shapes

Linkage

chemical bond is a link between 2 atoms to give a molecule

provides energy necessary to form a chemical

strength of the bond depends on the molecules involved in the process of bond formation

the chemical bond is composed by 2 electrons coming from the outer layer of each different atom to make a pair of electronds

shared electrons=pair of electrons

Membranes,
Energy,
and Cell Communication

Energy

the ability to do work

Kinetic Energy

energy of motion

e.g. muscle contractions

e.g. light energy

Potential Energy

stored energy available to do work

e.g gravitational energy

e.g. chemical energy

it is used, stored, and transformed in living systems

metabolism: the totality of the chemical reactions of an organism's body

a starting molecule is converted into a product through the used of intermediates

catalyzed by specific enzymes suited for the reaction

enzymes help to lower the energy barrier which reactant need to overcome before they can form products

substrate: the small molecule that an enzyme binds to

active site: where on the enzyme the substrate binds to

binding of a susbtrate forms weak bonds, changing the shape of the enzyme

weak bonds include hydrogen bonds and ionic bonds

regulation of enzyme function

inhibition of enzyme activity

competitive inhibition: a competitive inhibitor mimics a substrate, competing for the active site

noncompetitive inhibition: a noncompetitive inhibitor binds to the enzyme away from the active site, changing its shape so that the active site functions much less effectively

allosteric regulation

chemical reactions can be broken down into two pathways

catabolic

breaking down complex molecules into simpler compounds, releasing energy

e.g. cell respiration

exergonic

spontaneous

anabolic

when simpler molecules are converted into complex molecules, consuming energy

e.g. photosynthesis

endergonic

nonspontaneous

it is constantly being changed from one type of energy to another

e.g. photosynthesis: when light energy (kinetic energy)is converted into chemical energy (potential energy) to transform it into glucose

6CO2 +6H2O -> C6H12O6 +6O2

products are provided to the mitochondria

goes through cellular respiration
C6H12O6+6O2 -> 6CO2+6H2O+ATP

C6H12O6+6O2 -> 6CO2+6H2O+ATP

products (minus ATP!) are used as reactants for photosynthesis

broken up into two stages

light reactions

converts light (photons) & H2O into chemical energy (ATP & NADPH) while producing O2 as a byproduct.

chemical energy will be used to power the Calvin Cycle

NADPH: electron donor

ATP: energy currency of the cell

renewable resource regenerated by the addition of a phosphate group to ADP

catabolic reactions in the cell power the phosphorylation of ADP

occurs in the thylakoid membrane/space

calvin cycle

uses CO2 & chemical energy to synthesize glucose

broken up into three stages

carbon fixation

catalyzed by enzyme Rubisco

G3P synthesis

synthesizes glucose

RuBP Regeneration

a series of enzymatic reactions driven by ATP

thermodynamics: the study of energy transformations

system: the matter under study

closed system

open system

surroundings: matter in the rest of the universe

first law of thermodynamics: energy can be transferred or transformed but it cannot be created nor destroyed

second law: every energy transfer or transformation increases the entropy of the universe

entropy (S): measure of disorder

Gibbs free energy (G): helps to predict the spontaneity (or lack thereof) of a reaction at constant temperature and pressure

ΔG=ΔH-TΔS

ΔG<0 implies that ΔStotal>0

spontaneous

exergonic

ΔG=0 implies that ΔStotal=0

ΔG>0 implies that ΔStotal<0

nonspontaneous

cell communication

sending and receives signals

local signaling

paracrine & synaptic

long distance signaling

hormonal signaling

two types of receptors

membrane receptors

G protein linked receptor

steps at reception:

signal molecule binds to GPCR

allows G protein to bind

causes GDP to be replaced with GTP

*G protein switch removes phosphate group from GTP to make GDP

active G protein activates enzyme

Tyrosine Kinase receptor

made of two polypeptides which dimerize when a signal molecule is bound to each polypeptide

each polypeptide takes a phosphate group from ATP and adds it to the other polypeptide

called autophosphorylation

Ion Channel receptor

intracellular receptors

transduction

second messenger

cyclic AMP (cAMP)

formed from ATP using Adenylyl Cyclase

converted to AMP by phosphodiesterase

Cell membranes

Phospholipids

glycerol

2 fatty acids

phosphate

basic component

selectively permeable

types of membrane transport

simple passive

facilitated diffusion

active transport

DNA Structure, Replication, Expression and Regulation

regulation

Replication mechanism

three major steps

opening of the double helix and separation of the strands

priming of template strands

assemply of new DNA segment

monomer

nucleotides

hydrogen bonds connect complementary nucleotides

DNA STRUCTURE

a double helix formed from two complementary strands of nucleotides held together by hydrogen bonds between G-C and A-T base pairs.

cytosine, guanine, thymine, adenine

DNA strand is used as template strand to create new complementary strand

hydrogen bonds connect complementary nucleotides