Functional and structural analysis of Amylases

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Adaptation by ___________

a

2. Understand biological concept

Enzyme activity/function

DNS assay

Maltose standard curve

Measuring amylase activity

Energy requirements

Role of enzymes in metabolism

Reaction rate

Factors that affect enzyme acity

Physical: temperature

Chemical: pH, salt

Structural analyses: Bioinformatics

Structural analyses: Bioinformatics

Primary

NCBI

Pepstat

a

Secondary

Clustal Omega

a

Multiple sequence alignment

a

Boxshade

a

Tertiary

PDB

4. Use quantitative data to explain biological concept

Subtopic

Remote version

r

Videos for remote versionIntroduction to enzymes’ role in biochemical reactions: a review of proteins/enzymes, the amylase enzyme, and the reaction it catalyzes.https://www.youtube.com/watch?v=V6wHXtO9klA2. Maltose standard curve: constructing and using this graph will allow you to determine amylase activity.https://youtu.be/KNMz0pSgYbk3. Testing your hypothesis on amylase activity: review of the scientific method, the experimental design, and data analysis to determine the effect of an environmental factor on amylase activity. https://youtu.be/_BacifzIP64

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1. Apply scientific process

Question

r

what is the effect of temp0erature on human amylase

Hypothesis

Prediction

Experimental design

Variables

Dependant

Independant

Experimental group

Controls

Positive

Negative

Results

Conclusion

3. Develop quantitative skills

Graphing

Excel

Equation

Manual

Calculating maltose concentration

Equation from excel generated grap

Determine concentrations from manual graph

Relationship between maltose concentration and amylase activity

3. Develop quantitative skill

5. Develop critical thinking skills

Lab report