Jan 2013

Plus de détails

the founder

par Daniela Medina

Sample

par Gillian Dickinson

The Epic Maze Game

par Alex Elk

Word-Suffix

par Anthony Yuan

January 2013

Results

Outcomes

Expectations

Stimulated cells

Higher involvement in myotube formation

Alignment, perpendicular to direction of strain

Control

Unlikely >1 human nuclei per myotube

Low degree of fusion (<5%)

Measures

Degree of differentiation

Presence of Myosin

Morphology

No of syncytial nuclei

Human nuclei

Murine nuclei

Size

Directionality

Methodology

Mechanical Stimulation

Uniaxial cyclic tensile strain

Flexcell setup

6 well plates or the new design?

Cell line development

C2C12

C2C12-LifeAct-RFP

C2C12-GFP

ASC-GFP

Cellular stains

Myosin Heavy Chain

Primary [1:500]: Rabbit-anti-myosin

Secondary (10+10 ul/cm2): Zenon Alexa Fluor 647 mouse-anti-rabbit IgG

Cytoplasm

Green Fluorescent Protein

Ex: 487; Em: 509

Human Nuclei

Nuclear envelope

Primary [1:2000]: Monoclonal rabbit-anti-human Lamin

Secondary [1:400]: Alexa Fluor 555 conjugated goat-anti-rabbit

All Nuclei

DNA

Hoechst 33342

Introduction

Future Aspects

Conclusion

Discussion