Cell

Structure

Organelles

Nucleus

DNA

Nucleotides

Transcription

upstream

promoter

RNA polymerase

RNA transcript

5' cap

pre-mRNA

Intron splicing

mRNA

poly-A tail

Translation

tRNA

anticodon

amino acid

Ribosomes

binding sites

Peptidyl site

Aminoacyl site

Exit site

mRNA binding site

polypeptide

large subunit

small subunit

Initiation

Upstream

Promoter

Eukaryotes

RNA polymerase I

RNA polymerase II

RNA polymerase III

RNA transcript

Prokaryotes

RNA polymerase

Downstream

Nuclear Pores

Nuclear Membrane

Nucleolus

Nuclear Envelope

Chromatin

Endoplasmic Reticulum

Rough ER

Smooth ER

Ribosomes

Apparatus

Lysosomes

Peroxisome

Centrosome

Cell Wall

Cytoskeleton

Microfilaments

Intermediate Filaments

Microtubles

Plasma Membrane

Vacoule

Microvilli

Chloroplast

Thylakoid Membrane

Pilus

Nucleoid

Chromosomal DNA

Cell Capsule

Cell Membrane

Mitochondria

Inter-membrane Space

Inner Membrane

Granum

Mitochondrial Matrix

Eukaryotes

Animal Cells

Lysed

Normal

Shriveled

Plant Cells

Plasmolyzed

Flaccid

Turgid

Isotonic

Photosynthesis

Cellular Respiration

Redox Reactions

Oxidation

Reduction

Cytosol

Fermentation

Lactice Acid

Muscle Cells

Alcohol

Ethanol

Yeast

Anaerobic Respiration

Aerobic Respiration

Glycolysis

Gluucose

Pyruvate

Pyruvate Oxidation

Citric Acid Cycle/ Krebs cycle

Acetyl CoA

FADH2

Oxidative Phosphorylation

Electron Transport Chain (ETC)

Complex 1

Proton Pumps

H+

Complex 2

Complex 3

Complex 4

Ubiquinone

Cytochrome C

ATP Synthase

Chemeiosmosis

Net 36-38 ATP per Glucose

ATP

Oxidative Phosphorylation

Electron Carrier

NADH

Electroins

ATP

Substrate-Level Phosphorylation

Splits H2O

Light Reactions

Solar Energy to Chemical Energy

NADP+

NADPH

Nature of Sunlight

Electromagnetic energy

Electromagnetic Radiation

Electromagnetic Spectrum

Visible Light

Light Receptors

Pigments

Absorb

Spectrophotometer

Absorption Spectrum

Chlorophyll a

Chlorophyll b

Reflect

Accessory Pigments

Carotenoids

Photoprotection

Electromagnetic Wavelengths

Wavelengths

Photons

Excited State

Fluorescence

Cyclic Electron Flow

Action Specrum

Photophosphorylation

ADP

ATP

O2

Calvin Cycle

Carbon Fixation

CO2

Carbohydrate

Sugar

G3P

Cycle 3 Times

Phases

Carbon Fixation

Reduction

Regeneration

Components

Biological Molecules

Lipids

Fatty acids

Saturated

Unsaturated

Proteins

Amino Acids

Primary Structure

Secondary Structure

Alpha helix

Beta sheet

Tertiary Structure

Quaternary Structure

Types

Integral Proteins

Trans-membrane Proteins

Types

Transport Proteins

Aquaporins

ATP Synthase

Nucleic Acids

nucleotides

Phosphate groups

Nitrogeneous base

ACGTU

nucleosides

Pentose sugar

Ribose

RNA

mRNA

rRNA

tRNA

Deoxyribose

Carbohydrates

monosaccharide

glucose

disaccharide

lactose

polysaccharide

Storage Polysaccharides

Starch

Amylose

Amylopectin

Glycogen

Cellulose

Prokaryotes

Communication

Signal Transduction pathway

G-Protein Coupled receptor

G-Protein

GTP

GDP

cAMP

Second messengers

Phosphodiesterase

Nucleus

Protein

Ribosomes

Cytoplasm

Kinsaes

Protein Kinase A

Phosphotases

Protein Kinase

Transcription factor

Cellular Response

Apoptosis

Growth

Protein

DNA

mRNA

RNA

Phosphorylation Cascade

Receptor tyrosine kinases

Tyrosines

Dimerization

ATP

ADP

Long Distance Signaling

Blood Vessel

Local Signal

Synapse

Receptors

Receiving Cell

Signaling Hormones

Plasmodesmata

Cell Junction

Tight Junctions

Gap Junctions

Signaling Molecule

First Messengers

Epinephrine

Growth hormone

Division

Mitosis

Prophase

Chromatin

Kinetochore

Prometaphase

Centrosomes

Anaphase

Cleavage Furrow

Cell Plate

Telophase

Metaphase

Centromeres

Meiosis

Prophase 1

Prometaphase 1

Metaphase 1

Anaphase 1

Telophase 1

Sister Chromatids

Transportation

Potassium Pump

Sodium Pump

Homologous Chromosomes

Sodium-Potassium Pump

Matter

Compounds

Ex) NaCl

Elements

Essential elements

Trace Elements

Ex) Copper

Atoms

Protons

Electrons

Energy

Free Energy

Exergonic

Endergonic

Energy Levels of Electrons

Potential Energy

Kinetic Energy

Thermal Energy

Chemical Energy

Laws of Energy

Thermodynamics

Absorb Energy

Lose Energy

Electron Orbitals

s, p, d, f

Valence Electron/Shells

Chemical Bonds

Covalent Bonds

Polar

Nonpolar

Attraction: Electronegativity

Ionic Bond

Cations

Anions

Hydrogen Bonds

Water

Temperature

Kinetic Energy

Thermal Energy

Heat

Calorie

High Specific Heat

Heat of Vaporization

Evaporative Cooling

Solvent

Aqueous Solution

Solute

Solution

Cohesion

Surface Tension

Adhesion

Reason Ice Floats on Water

Van Der Waals

Chemical Reactions

Break Bonds

Make Bonds

Metabolic Pathway

Catabolic

Anabolic

Up to 8 Electrons

Neutrons

Isotopes

Radioactive Isotopes

Carbon

6 Electrons

Carbon Skeletons

Length

Branching

Double Bond Position

Rings

Hydrocarbons

Atomic Nucleus

Atomic Number

Atomic Mass

Bonds

Single Bond

Triple Bond

Double Bond

Molecules

Molecular Shape/Function

Organic Molecules

CHONPS

Variation

Structural Isomers

Cis - Trans Isomers

Enantiomers

Polar Molecule

Nonpolar Molecule

Chemical Groups

Hydroxyl

-OH

Carbonyl

>C=O

Carboxyl

-COOH

Amino

-NH2

Sulfhydryl

-SH

Phosphate

-OPO3 2-

ATP

Adenosine

3 Phosphates

Methyl

-CH3

Floating topic

Acids and Bases

Hydrogen Ion (H+)

Hydroxide Ion (OH-)

PH

Scale

Acidic

<7

Neutral

=7

Basic

>7

1-14

Acid

PoH

Base

Buffers

Ocean Acidification

Hypertonic

Hypotonic

First Law

Second Law

Entropy

Spontaneity

Delta G is Negative

Non-spontaneity

Delta G is Positive

Delta G

Free Energy

Delta G = Delta H - T (Delta S)

Delta S: Change in Entropy

Delta H: Enthalpy

T: Temperature (K)

Flagella

ATP

Chemical Work

Transport Work

Mechanical Work

Hydrolysis

Energy Coupling

Phosphorylated Intermediate

Enzymes

Catalysts

Activation Energy

Substrates

Temperature

Cofactors

Coenzymes

Inhibitor

Normal Binding

Competitive Inhibition

Noncompetitive Inhibition

Allosteric Activation

Cooperativity

Feedback Inhibition

Active Site

6CO2 + 12H20 + Light E --> C6H12O6 + 6O2

Photosystems

Reaction-center complex

Light-Harvesting complex

Primary Electron Acceptor

Photosystem II

Electron Transport Chain

Photosystem I

Linear Electron Flows

Phospholipid Bilayer

Polar

Hydrophillic

Non-Polar

Hydrophobic

Chloroplast

Mesophyll

Stomata

Stoma

Stroma

Thylakoids

Chlorophyll

Thylakoid Space

DNA sequencing

DNA sequence is denatured

Synthesis of new strands

Labeled strands are separated

Next Generation sequencing

Genomic DNA is fragmented

Fragment isolated

Fragment is copied

Bead placed into a small well to hybidize

In each well, nucleotide is joined

Nucleotide is washed off

Process is repeated

DNA Cloning

Gene inserted into plasmid

Plasmid put into bacterial cell

Host cell grown to form clone of cells

Genes used in different ways

Recombinant DNA

Restriction enzyme cuts sugar phosphate backbones

DNA fragment from another source is added

DNA ligase seals the strands

Recombinant Plasmid

Polymerase Chain Reaction

Denaturation

Annealing

Extension

Cycle 2

Cycle 3

Cloning

Stem cell

Embryonic and Adult Stem Cells

iPS

Pluripotent

Plants

Totipotent

Animals

Dolly the lamb