Kategorier: Alle - bacteria - microorganisms - soil

av Pablo López 5 år siden

289

Methodology

The experimental process involves incubating specific microorganisms over a period of 42 days, with periodic sampling conducted every 7 days. The microorganisms, including Aeromonas, Micrococcus, and Serratia, are mixed to form a bacterial culture, which is then combined with lubricating engine oil.

Methodology

Methodology

The soil is analyzed

heterotrophic bacteria
pH
Phosphorus
Nitrogen
Organic carbon

Used lubricating engine oil used OTAL QUARTZ 5000 with SAE grade 20W-50

Then the soil samples are dried and homogenized.

Agricultural land is collected from automotive workshop in Ogbomoso, Oyo state

Kinetic Modelling and Half-Life Study on Bioremediation of Soil Co-Contaminated with Lubricating Motor Oil and Lead Using Different Bioremediation Strategies

The information is analyzed by ANOVA, at a level of 9 <0.05.

Periodic sampling of microorganisms is performed every 7 days for 42 days.

The microorganisms are incubated for 42 days.

Biodegradation of engine oil is expected.

Then it goes through a centrifuge

48 are expected to grow and then 1 ml of the culture is introduced at a concentration of lubricating engine (10% p/v)

The mixed bacterial culture (100 ml) is mixed with lubricating oil (3% p/v).

The flasks are prepared with Aeromonas, Micrococcus and Serratia, to mix in a sterilized test tube. A mixed bacterial culture is obtained.

The microorganisms for bioaugmentation were isolated.