Methodology
The microorganisms for bioaugmentation were isolated.
The flasks are prepared with Aeromonas, Micrococcus and Serratia, to mix in a sterilized test tube. A mixed bacterial culture is obtained.
The mixed bacterial culture (100 ml) is mixed with lubricating oil (3% p/v).
48 are expected to grow and then 1 ml of the culture is introduced at a concentration of lubricating engine (10% p/v)
Then it goes through a centrifuge
Biodegradation of engine oil is expected.
The microorganisms are incubated for 42 days.
Periodic sampling of microorganisms is performed every 7 days for 42 days.
The information is analyzed by ANOVA, at a level of 9 <0.05.
Kinetic Modelling and Half-Life Study on Bioremediation of Soil Co-Contaminated with Lubricating Motor Oil and Lead Using Different Bioremediation Strategies
Agricultural land is collected from automotive workshop in Ogbomoso, Oyo state
Then the soil samples are dried and homogenized.
Used lubricating engine oil used OTAL QUARTZ 5000 with SAE grade 20W-50
The soil is analyzed
Organic carbon
Nitrogen
Phosphorus
pH
heterotrophic bacteria