Functional and structural analysis of Amylases

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The epistemological foundations of quantitative research

av WENDY DAYANA CONDOR SARAGOSIN

Cost

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Animal systems

av Virina Maged Atia Malty

Functional and structural analysis of Amylases

5. Develop critical thinking skills

Lab report

3. Develop quantitative skills

3. Develop quantitative skill

Relationship between maltose concentration and amylase activity

Calculating maltose concentration

Determine concentrations from manual graph

Equation from excel generated grap

Graphing

Manual

Excel

Equation

1. Apply scientific process

Conclusion

Results

Experimental design

Controls

Negative

Positive

Experimental group

Variables

Independant

Dependant

Prediction

Hypothesis

Question

what is the effect of temp0erature on human amylase

Remote version

Videos for remote version

Introduction to enzymes’ role in biochemical reactions: a review of proteins/enzymes, the amylase enzyme, and the reaction it catalyzes.

https://www.youtube.com/watch?v=V6wHXtO9klA

2. Maltose standard curve: constructing and using this graph will allow you to determine amylase activity.

https://youtu.be/KNMz0pSgYbk

3. Testing your hypothesis on amylase activity: review of the scientific method, the experimental design, and data analysis to determine the effect of an environmental factor on amylase activity. https://youtu.be/_BacifzIP64

4. Use quantitative data to explain biological concept

Subtopic

2. Understand biological concept

Structural analyses: Bioinformatics

Tertiary

PDB

Secondary

Boxshade

Multiple sequence alignment

Clustal Omega

Primary

Pepstat

NCBI

Factors that affect enzyme acity

Chemical: pH, salt

Physical: temperature

Reaction rate

Energy requirements

Role of enzymes in metabolism

Enzyme activity/function

Measuring amylase activity

Maltose standard curve

DNS assay

Adaptation by ___________