Kategorier: Alle - bacteria - microscope - measurement

af Kohilavaani Ganesan 7 år siden

545

Microscopy Mind Map

The text delves into various microscopy techniques and their applications in studying microorganisms. It highlights the distinction between gram-positive and gram-negative bacteria based on cell wall structures, explored through differential staining methods such as acid-fast staining and gram staining.

Microscopy Mind Map

Acid-fast Staining

Gram Staining

Divides bacteria into two based on difference in cell wall structures

Gram Negative

Gram positive

MICROSCOPY

UNITS OF MEASUREMENT

Metric System
Micoorgansims and their structural components are measured in micrometers, nanometers and angstroms

Preparations of Specimens for Light Microscopy

Preparing Smears
Fixation

Staining

Smears
Wet Mount
Demonstrating motility of microorgansims

Electron Microscope

To study the surface features of cells and viruses
Employs a beam of electron in place pf light wave to produce magnified image

Fluorescence Microscope

Applications in medical microbiology
Specimens usually stained with flourochromes
Expose specimen to ultraviolet, violet or blue light

Phase Contrast Microscope

Stain is not necessary
Excellent way to observe living things
Converts differences in refractive index

Dark Field Microscope

Used to identify bacteria
Used to observe internal structures in eukaryotic micoorganisms
Produce a bright image of the object

Creates a sharp 3D image bybusing laser beam nd numerous applications including study of biofilms

Confocal Micoscopy

UV Microscope

Has shorter wavelength

Permits greater resolution

Microscope Parts

Body Tube
Objectives
Nosepiece
Substage
Stage Clips
Stage
Fine Adjustment Knob
Coarse Adjustment Knob
Light Source
Arm
Base

Special Staining

Negative Staining
Endospore Staining

Flagella Staining

Types of Staining

Differential Staining

Microscope is an instrument to observe microorgnisms

Light Microscope and Electron Microscope
Subtopic