Methodology

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Chapter 12

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Methodology

The soil is analyzed

heterotrophic bacteria

pH

Phosphorus

Nitrogen

Organic carbon

Used lubricating engine oil used OTAL QUARTZ 5000 with SAE grade 20W-50

Then the soil samples are dried and homogenized.

Agricultural land is collected from automotive workshop in Ogbomoso, Oyo state

Kinetic Modelling and Half-Life Study on Bioremediation of Soil Co-Contaminated with Lubricating Motor Oil and Lead Using Different Bioremediation Strategies

The information is analyzed by ANOVA, at a level of 9 <0.05.

Periodic sampling of microorganisms is performed every 7 days for 42 days.

The microorganisms are incubated for 42 days.

Biodegradation of engine oil is expected.

Then it goes through a centrifuge

48 are expected to grow and then 1 ml of the culture is introduced at a concentration of lubricating engine (10% p/v)

The mixed bacterial culture (100 ml) is mixed with lubricating oil (3% p/v).

The flasks are prepared with Aeromonas, Micrococcus and Serratia, to mix in a sterilized test tube. A mixed bacterial culture is obtained.

The microorganisms for bioaugmentation were isolated.