Gene expression in the Three-Spined Stickleback (Gasterosteus aculeatus) of Marine and Freshwater Ecotypes

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Study guide

luonut Diego Hernández de la Torre

Gene Regulation

luonut Alana Beard

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luonut Briana I

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luonut Steve Dsyon

Gene expression in the Three-Spined Stickleback (Gasterosteus aculeatus) of Marine and Freshwater Ecotypes

the Three-Spined Stickleback

Samples of marine three-spined stickleback (hereinafter referred to as “M”) were collected from:

Freshwater samples were collected from the Machinnoye Lake, not far from the village of Tchkalovsky, Loukhskiy district, Republic of Karelia.

The White Sea, near the N.A. Pertsov White Sea Biological Station of Moscow State University.

A marine population of three-spined stickleback usually uses freshwater streams and lakes for spawning

Gene expression

The RNA concentration for each sample was determined using a BioAnalyzer 2100 (RNA 6000 Nano Kit) (Agilent, USA)

The total number of reads of 75 nucleotides in length was 85438630 and 110690898 in the libraries from the marine and freshwater samples, respectively.

To identify the genes which are differentially expressed in the samples of threespined stickleback, the Illumina nucleotide reads were mapped on the G. aculeatus reference genome from the Ensembl database.

Differential expression was established using the edgeR package, which calculates the variance of the expression index for each gene.

In total, 2,982 differentially expressed genes between marine and freshwater populations were discovered