PCR (Polymerase chain reaction)

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PCR (Polymerase chain reaction)

Name the character


Type in the name of the character whose change throughout the story you are going to analyze.

Example: Nick Carraway.

Digital PCR as a tool to measure the persistence of HIV

The virus persists and recovers when treatment is stopped

Persistent virus must be quantifiable

digital PCR is gaining popularity

Various commercial digital PCR platforms available

advantage

Future Advances in Digital PCR Technology Show Promising

to aid in the accurate quantification and characterization of the persistent HIV reservoir

Disadvantages

reliable segregation between positive and negative droplets continues to be questioned

Currently available digital PCR platforms occasionally struggle with unexplained false positive partitions

But Bio-Rad's QX200 ddPCR is currently the most widely used platform in HIV research.

Used real-time PCR (qPCR) ic

Other types of PCR

Character's behavior

Think of the character's behavior at the beginning of the story and look for the way it changed throughout the story.

Nested PCR

PCR in situ

Multiplex PCR

Quantitative PCR

RT-PCR

Digital PCR

has a variant called DDPCR

a luminous detector is attached,

The flow comes out in the form of bubbles

Use a two micro fluid systems one at the beginning and one at the end of the process

has four steps

Initial behavior

What is the character's behavior at the beginning of the story? Type in a relevant quote for your statement.

Example: Nick seems to be an honest person, calling himself 'one of the few honest people that I have ever known'.

Obtaining data

PCR amplification

The division or distribution

sample preparation

absolute quantification

Initial behavior

How does the character act at the beginning of the story? Type in a relevant quote for your statement.

Example: Nick shows his immature side as he leaves to New York in order to avoid 'being rumored into marriage' with his girlfriend.

What is needed to do a PCR?

Character's feelings

Focus on the way the character's feelings are presented at the beginning and at the end of the story, while explaining why they have changed.

Thermal cycler

A thermal cycler is a device that regulates the temperature in each PCR cycle

Buffer solution

A buffer solution is a solution that is capable of regulating the pH, that is, the acidity or basicity conditions, of our PCR

Divalent magnesium ions

Positively charged ions are used as cofactors of the polymerase. These cations are essential for the function of DNA polymerase. Magnesium chloride is normally added so that magnesium is released upon dissociation

DNA polymerases

The most widely used given its effectiveness, is the DNA polymerase of the bacterium Thermus aquaticus, also called Taq Polymerase

Primers

are oligonucleotides, short DNA sequences, that bind to the template DNA molecule and serve as a starting point to begin DNA synthesis

The reason for the change of feelings

What caused the character to change the first belief you mentioned? Type in the reason for the change.

Example: disgust towards the lack of morality in the Buchanan family.

Deoxyribonucleotides-triphosphate

DNA is made up of 4 types of nucleotides, made up of 4 nitrogenous bases: adenine, guanine, cytosine, and thymine. It is expected, then, that these 4 deoxyribonucleotides-triphosphate are essential to be able to obtain new DNA molecules.

Change of feelings

In what way did the character change the feeling you mentioned?

Type in a quote to support your statement.

Example: 'They are a rotten crowd. You're worth the whole damn bunch put together.' - Nick criticizing the Buchanans.

Template DNA

DNA fragment What do we want to amplify by PCR

Initial feelings


How does the character feel about a certain subject at the beginning of the story? Type in a relevant quote to support your statement.

Example: "Reserving judgements is a matter of infinite hope."

Technique used in molecular biology that allows to get a large number of copies of a DNA fragment, starting from a tiny amount of this biomolecule

Title


Type in the title and author of the literary work that introduces the character.

Example: The Great Gatsby, by F. Scott Fitzgerald.