Gram Stain

Meer zoals dit

Food Enzyme

door Pei Xin Wong

War

door Breanna Kargle

Domain Bacteria

door Holly schomaker

Flow Chart

door Anna Xu

Gram Stain

Gram Negative (Pink)

MAC Test

Result: Colorless colonies (negative result for lactose fermentation)

Result: Colorless colonies (coloration of medium) (Non-lactose fermentation)

Result: Colorless colonies (Non-lactose fermentor)

Result (indicated by Slant/Butt/Gas/H2S): K/A/v/- -Yellow/acidic = A -Pink/alkaline = K -Gas bubble in medium = + CO2/H2 production -Black precipitation = + for H2S production -Variable results = V

Yersinia enterocolitica

Result: Colorless colonies with black centers (Non-lactose fermentor, but the black centers suggest H2S gas production)

Result (indicated by Slant/Butt/Gas/H2S): K/A/-/+ -Yellow/acidic = A -Pink/alkaline = K -Gas bubble in medium = + CO2/H2 production -Black precipitation = + for H2S production -Variable results = V

Salmonella enterica

Result: Bright pink colonies (positive result for lactose fermentation)

EMB Test

Result: Dark Purple color on medium (positive result for lactose fermentation)

Result = Positive (Indicated by bright blue color change)

Klebsiella pnemoniae

Result: Metallic Green color on medium (positive result for lactose fermentation)

SS Test

Result: Mostly inhibited (but can have some pink colonies) -if pink, indicates lactose fermentor

TSI Slant Test

Result (indicated by Slant/Butt/Gas/H2S): A/A/v/- -Yellow/acidic = A -Pink/alkaline = K -Gas bubble in medium = + CO2/H2 production -Black precipitation = + for H2S production -Variable results = V

Citrate Test

Result = Negative (Indicated by no color change from green)

Escherichia coli

Gram Positive (Purple)

Cocci

Catalase Test

Negative Result= No bubbling (organism does not produce catalase)

Result: Inhibited (cannot withstand high salt concentration)

Result: α-hemolysis (greenish halo around colonies)

Streptococcus pneumoniae

Streptococcus pyogenes

Positive Result = Bubbling (organism produces catalase)

MSA Test

Result: Growth, no color change. (cannot ferment mannitol)

Result: Non (y) (no change to surrounding medium)

Staphylococcus epidermis

Result: Growth, yellow medium. (can ferment mannitol)

BAP Test

Result: β-hemolysis (clear halo surrounding colonies)

Staphylococcus aures

Rod