Gram Stain

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Bacteria

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Gram Stain

Gram Negative (Pink)

MAC Test

Result: Colorless colonies (negative result for lactose fermentation)

Result: Colorless colonies (coloration of medium) (Non-lactose fermentation)

Result: Colorless colonies (Non-lactose fermentor)

Result (indicated by Slant/Butt/Gas/H2S): K/A/v/- -Yellow/acidic = A -Pink/alkaline = K -Gas bubble in medium = + CO2/H2 production -Black precipitation = + for H2S production -Variable results = V

Yersinia enterocolitica

Result: Colorless colonies with black centers (Non-lactose fermentor, but the black centers suggest H2S gas production)

Result (indicated by Slant/Butt/Gas/H2S): K/A/-/+ -Yellow/acidic = A -Pink/alkaline = K -Gas bubble in medium = + CO2/H2 production -Black precipitation = + for H2S production -Variable results = V

Salmonella enterica

Result: Bright pink colonies (positive result for lactose fermentation)

EMB Test

Result: Dark Purple color on medium (positive result for lactose fermentation)

Result = Positive (Indicated by bright blue color change)

Klebsiella pnemoniae

Result: Metallic Green color on medium (positive result for lactose fermentation)

SS Test

Result: Mostly inhibited (but can have some pink colonies) -if pink, indicates lactose fermentor

TSI Slant Test

Result (indicated by Slant/Butt/Gas/H2S): A/A/v/- -Yellow/acidic = A -Pink/alkaline = K -Gas bubble in medium = + CO2/H2 production -Black precipitation = + for H2S production -Variable results = V

Citrate Test

Result = Negative (Indicated by no color change from green)

Escherichia coli

Gram Positive (Purple)

Cocci

Catalase Test

Negative Result= No bubbling (organism does not produce catalase)

Result: Inhibited (cannot withstand high salt concentration)

Result: α-hemolysis (greenish halo around colonies)

Streptococcus pneumoniae

Streptococcus pyogenes

Positive Result = Bubbling (organism produces catalase)

MSA Test

Result: Growth, no color change. (cannot ferment mannitol)

Result: Non (y) (no change to surrounding medium)

Staphylococcus epidermis

Result: Growth, yellow medium. (can ferment mannitol)

BAP Test

Result: β-hemolysis (clear halo surrounding colonies)

Staphylococcus aures

Rod