Kategorier: Alla - toxins - control - dairy - contamination

av Puteri Nur' Ain för 8 årar sedan

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BIOPROCESS

Aflatoxin M1 contamination in dairy products, such as milk, cheese, and yogurt, varies based on several factors including the type of product, manufacturing processes, and seasonal conditions.

BIOPROCESS

CONTAMINATION OF DAIRY PRODUCTS BY AFLATOXIN M1 AND ITS CONTROL BY PROBIOTICS

CONCLUSION AND RECOMMENDATION

Apply ideal recommend limit to minimize the AFM1 contamination in milk
Widespread and continuous training for both the producers and consumers
Maintain low levels of AFB1 in the feeds of dairy animals
Milk and dairy product have to be controlled continuosly

RESULT

Concentration of AFM1 in yoghurt depends on some variable
country and season
condition of yoghurt ripening
type of yogurt
manufacturing procedures
Concentration of AFM1 in cheese depends on some variable
production technologies
water content
type of cheese
The results obtain depends on the country
Aflatoxin mostly found in raw milk samples
Mozarella cheese have the highest rate exceeding the permissible rate
1 out of 9 isolates of A.flavus produced aflatoxin

Probiotics use

Play important role in digestive and respiratory
Bifidobacterium lactis
more ability to remove AFM1
involves physical binding of the toxin to the bacterial cell wall
AFM1 bind to milk protein
Example
Bifidobacterium
Lactobacillus
reducing the availability of AFs

METHOD

Detoxification of AFM1 in Yoghurt by Lactic acid Bacteria
Binding ability of LAB in AFM1 contaminated milk
Preparation of lactic acid bacteria inoculum
Cultures activation
ELISA TEST
Statistical Analysis
AFM1 evaluation
Sample preparations
AFM1 determination
Determination of toxigenic of A.flavus
Isolation and identification of moulds
Preparation of sample

AFLATOXIN M1

Possible accumulation and linkage to DNA
risk more to the infants
Relatively stable during pasteurization and storage
Not destroyed
Found when lactating animals are fed with contaminated feedstuff
The hydroxylated matabolite of aflatoxin B1
Less carcinogenic and mutagenic than AFB1

AFLATOXIN

It also can cause liver damage and cancer
Contamination occur from
storage
handling
harvest
field
It can grow under favourable temperature and humidity
Highly toxic produced by the species of Aspergillus
A. parasiticus
A. flavus