作者：Aneesh Risbud 5 年以前
254
CELL DISRUPTION
The text delves into various physico-mechanical and chemical methods for cell disruption, which is crucial for processes like enzyme purification. Physico-mechanical techniques include agitation with abrasives, using rotating discs and beads made of materials such as glass, alumina ceramics, and titanium, often cooled by a jacket to manage heat dissipation.
開啟
CELL DISRUPTION CHEMICAL METHODS ENZYME TREATMENT USED FOR [RETREATMENT EXPENSIVE AND PRESENCE COMPLICATE DOWNSTREAM HYDROLYZE SPECIFIC BONDS BY ENZYMES TRICODERMA
PENICILLIUM
MICROMONOSPORA
LEUCOCYTES
STREPTOMYCES
OSMOTIC SHOCK EFFECT IS MINIMAL CAUSES SUDDEN CHANGE IN SALT CONC. DETERGENTS AND ALKALI TREATMENTS ALKALI ENZYME
20-30 MINS
pH- 11.5-12.5
HYDROLYSIS OF CELL WALL
DETERGENTS PROTEIN DENATURATION
COMPOUNDS
TRITON X-100
SODIUM LAURYL SULPHATE
SDS
QUART AMMONIUM
DAMAGE LIPOPROTEIN
PHYSICO-MECHANICAL METHODS ULTRASONICATION VOLUMES UPTO 10 CM3 ARE EFFECTIVE PROBES SHORT RANGE
SHORT WORK LIFE
20 kHz VIBRATION LEADING TO CAVITATION AND SHOCK WAVES FREEZE THAWING SLOW , LIMITED RELEASE OF CELLULAR MATERIALS THAWING CAUSES DISRUPTION OF MICROBIAL CELL PASTE- ICE CRYSTAL FORMATION AGITATION WITH ABRASIVES DISSIPATION OVERCOME BY COOLING JACKET BEADS- GLASS, ALUMINA CERAMICS AND TITANIUM ROTATING DISCS AND SMALL BEADS IN A DISINTEGRATOR SOLID SHEAR DISRUPTION DUE TO ICE CRYSTALS IDEAL FOR TEMPRATURE LABILE PRESSURE EXTRUSION THROUGH ORIFICE AT -25 C LIQUID SHEAR WORKS ON PRINCIPLE OF PRESSURE DROP ACROSS VALVES IMPINGES AGAINST OPERATIVE PRESSURE HIGH PRESSURE HOMOGENIZERS EFFECTIVE FOR MICROBIAL DISRUPTION LARGE SCALE ENZYME PURIFICATIOM SLURRY PASSES THROUGH NO RETURN VALVES
