CELL DISRUPTION

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Metabolism

luonut True Jarrel [STUDENT]

Organic molecules concept map

luonut Emily Kroening

B1 Cell Level Systems - KW

luonut Lynne Hann

Digestion

luonut Woon Kiat

CELL DISRUPTION

CHEMICAL METHODS

ENZYME TREATMENT

USED FOR [RETREATMENT

EXPENSIVE AND PRESENCE COMPLICATE DOWNSTREAM

HYDROLYZE SPECIFIC BONDS BY ENZYMES

TRICODERMA

PENICILLIUM

MICROMONOSPORA

LEUCOCYTES

STREPTOMYCES

OSMOTIC SHOCK

EFFECT IS MINIMAL

CAUSES SUDDEN CHANGE IN SALT CONC.

DETERGENTS AND ALKALI TREATMENTS

ALKALI

ENZYME

20-30 MINS

pH- 11.5-12.5

HYDROLYSIS OF CELL WALL

DETERGENTS

PROTEIN DENATURATION

COMPOUNDS

TRITON X-100

SODIUM LAURYL SULPHATE

SDS

QUART AMMONIUM

DAMAGE LIPOPROTEIN

PHYSICO-MECHANICAL METHODS

ULTRASONICATION

VOLUMES UPTO 10 CM3 ARE EFFECTIVE

PROBES

SHORT RANGE

SHORT WORK LIFE

20 kHz VIBRATION LEADING TO CAVITATION AND SHOCK WAVES

FREEZE THAWING

SLOW , LIMITED RELEASE OF CELLULAR MATERIALS

THAWING CAUSES DISRUPTION

OF MICROBIAL CELL PASTE- ICE CRYSTAL FORMATION

AGITATION WITH ABRASIVES

DISSIPATION OVERCOME BY COOLING JACKET

BEADS- GLASS, ALUMINA CERAMICS AND TITANIUM

ROTATING DISCS AND SMALL BEADS IN A DISINTEGRATOR

SOLID SHEAR

DISRUPTION DUE TO ICE CRYSTALS

IDEAL FOR TEMPRATURE LABILE

PRESSURE EXTRUSION THROUGH ORIFICE AT -25 C

LIQUID SHEAR

WORKS ON PRINCIPLE OF PRESSURE DROP ACROSS VALVES

IMPINGES AGAINST OPERATIVE PRESSURE

HIGH PRESSURE HOMOGENIZERS EFFECTIVE FOR MICROBIAL DISRUPTION

LARGE SCALE ENZYME PURIFICATIOM

SLURRY PASSES THROUGH NO RETURN VALVES