Categorías: Todo - transcription - proteins - translation - enzymes

por Allison Schellenberg hace 4 años

482

Concept Map (Fall 2020)- Group #7

The process of gene expression involves several key stages, starting with transcription where pre-mRNA is synthesized and subsequently modified. Introns are spliced out, and a 5' guanine cap along with a 3'

Concept Map (Fall 2020)- Group #7

Eukaryotic cells

Plant Cell

Golgi body
Vesicles
-Central Vacuoles: serve as repository for inorganic ions (found in mature plant cells)
Chloroplasts
Stroma (internal fluid)

equivalent of matrix in mitochondria

Inner membrane spaces
Thylakoid

synthesize proteins

stores genetic material

Granum

stack of thylakoid

Animal Cell

Mitochondria
Production of ATP by breaking down complex molecules

intermembrane space

Matrix

Inner membrane (folded into cristae)

DNA

free ribosomes

make proteins

Outside of ER/ nuclear envelope: bound ribosomes

Cytosol: free ribosomes

Complexes of rRNA and protein
Endomembrane system
Endoplasmic Reticulum

Continuous with nuclear envelope

Smooth ER

-No attached ribosomes -Synthesizes lipids -Metabolizes carbs -Detoxifies drugs and poison -Stores Calcium ions

Rough ER

-Surface studded with ribosomes -Bound ribosomes secrete glycoproteins -Distribute transport vesicles -Known as membrane factory of cell

Vacuoles

-Food Vacuoles: when cells engulf food or other particles -Contractile Vacuoles: pumps excess water out of cell (found in freshwater protists)

Lysosomes

-packed with enzymes -function in acid pH -H+ pumped to maintain low pH

Phagocytosis

Golgi Apparatus

-Made of different sacs -Vesicles transported from ER to out of Golgi apparatus

Nucleus
Double membrane: Nuclear envelope

Lamina: lines in the inner surface that protect the integrity

Pores: help transport molecules in and out of cell

Nucleolus

Site of rRNA synthesis

- organized into chromosomes - DNA + Proteins = chromatin

ORIGINS OF LIFE

pre-mRNA--> mRNA

Introns spliced out
Alternative splicing

1 gene codes for 1+ proteins

5' guanine cap

3' polyA tail

Protein P Phosphotase (PP)

Eukaryotic Transcription

Transcription Factors
Specific

Attach to Enhancer

General

Attach to promoter/proximal control element

Promoter
Transcription Initiation Complex

pre-mRNA

Control Element
Distal

Enhancer

Increased Expression

Proximal

Basal Level Expression

Low level of protein production

Prokaryotic Transcription

Operon
Structural Genes
promoter

RNA Polymerase

operator

Glucose present

Activator

Turns gene expression on

Repressor

Turns gene expression off

Cell Signaling

Reception

Relay Molecule
Transduction

Nuclear Response Pathway

Receptor Activated

Receptor activates phosphorylation Cascade

Activation of a Transcription Factor

Transcription

Gene Expression

G-Protein and cAMP Pathway

G-Protein Coupled Receptor (GPCR)

Inactive G-Protein

Activated G-Protein

Adenylyl Cyclase

cAMP Formed

AMP Formed

Protein Kinase A

Phosphorylation Cascade Pathway

Signal Molecule (Ligand)
Inactive Receptor -> Active Receptor

Relay Molecule is Releases

Inactive Protein Kinase 1

Active Protein Kinase 1

Inactive Protein Kinase 2

Active Protein Kinase 2

Inactive Protein Kinase 3

Active Protein Kinase 3

Inactive Protein

Active Protein

Cellular Response

G-Protein Pathway

pre-mRNA processing

Slicing of introns (Eukaryotes)

Translation

Ribosome

Free vs Bound
Bound

protein moves to Rough ER

transported via vesicle

moves to golgi

moves through plasma membrane

Free

universal starting point

P, A, E site
peptidyl transferase

polypeptide growth

hydrolysis of GTP
proteins
rRNA
ribozyme function

mRNA

from transcription
codons

wobble

tRNA

amino acids

aminoacyl-tRNA synthetase

anticodons

Eukaryote

Prokaryote

Bacteria

Cell wall (Peptidoglycan) Unique to bacteria
protects cell, provides support, maintains cell shape

Archaea

Form endospores
helps to remain alive in harsh conditions

Harsh chemicals

Extreme drying

Extreme temperatures

Bacteria & Archaea

Flagella
Locomotion (movement)
Pili
transfers DNA from one cell to another
Fimbriae
able to stick to each other
Plasma membrane (Lipid Bilayer)
Contains NO membrane bound organelles
Contains NO Nucleus
Ribosomes

Protein synthesis

Plasmid

DNA that is separate from chromosomes

Cytoplasm

Jelly fluid within cell

Nucleoid

region where cell's DNA is located

Glycocalyx
protect against dehydration

Slime layer

Avoids phagocytosis from WBCs

Capsule

Sticky layer