カテゴリー 全て - glucose - transcription - cholesterol - camp

によって Graciela Aguilar 7年前.

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Sample Mind Map

The provided text outlines various biological concepts and cellular mechanisms. It discusses the role of lactose and glucose in cellular metabolism, highlighting their influence on cyclic AMP levels.

Sample Mind Map

Feedback inhibition

inhibition via products response

ECM

Peripheral Protein

Glycoprotein

Glycolipid

Microfilaments

Integral Protein

Cytoskeleton

Polarr

Ionic

Hydrophobic

Hydrogen Bonding

Van der Wals

Covalent

Domains of life

Archea

No nuclear envelope

Chloroplast

Lysosomes

Peroxisomes

Mitochondrin

Floating topic

Vacuole

Plasma Membrane

Cell Wall

Golgi

ER

Ribosomes

Nucleus

Bacteria

No membrane enclosed organelles

Peptidoglycan

membrane lipids

Animal

Organelles

Functional Groups

Sulfhydryl

Amino

Carbonyl

Methyl

Hydroxyl

Carboxyl

removes

Phosphatase

Adds

Phosphate

Kinase

Ligand

Open

Dimerization

Signal molecules

Reception

Signal

G protein active

GTP displaces GDP
active enzyme

no singal

G protein inactive

Inactive enzyme

Diffuse

NUcleus
Cytoplasm

Nnonpolar

Cell Membrane

TKR

Ion channel

GPCR

Receptor

Reponse

Transcription

Hormone

Synaptic

Pacarine

target

long-distance

Local

Cell communication

Direct Contact

Cell Singaling

Requires

Coupled

Contain

Down gradient

Nonpolar

Small

Across concentration

Down concentration

High temps.

prevents extreme fluidity

Cold temps.

Unsaturated tails needed

prevents tight packing/ soldification

Gradient

ATP sythentase

Powers

Cotransporters

Allows passage of

Hydrophobic exterior/ hydrophilic interior

Hydrophilic

Charged

Polar

Large

Amphipathic

regulates membrane fluidity

Affects fluidity

Requires Energy

Across Gradient

Active Transport

Main Ingredients

Carbs

Affects Membrane

Phospholipid membranes

Work possible

Proton Pump

Electrogenic pump

Relative to cell

Plasmolyzed
Turgid
Ideal conditions
Flaccid

Animals

Hypertonic

Lyse

Hypotonic

Shrivel

Isotonic

Normal

Plant

Tonicity

Maintain concentrations- substance/solute

facilitiated diffusion

Simple diffusion

Active transport

OR

Affinity for

Na

K

Na/K pump

Channel

Carrier

Two conformations

Carbohydrate markers

Uneven distribution of carbs.

Glycoproteins

Glycolipids

Functions include:

Are examples of

Corepressor

Accessible for modification

Amino acid tails exposed

Opens up chromatin

Closes chromatin

Proximal

Distal

control elements

General transcription factors

Specific transcription factors

Acetlyation

Methylation

DNA organized neatly in nucleus

Allows coiling of DNA

Histone packaging

Eukaryotes

Tryp. present

Tryp. absent

Repressor not bound

Protein

ECM/ cytoskeleton

Inter cellular joining

signal transduction

Cell cell recognition

Transport

Enzymatic

Polypeptide subunits

Repressor bound

Glucose high

CAMP low

When conditions are met

Lactose absent

repressor unbound

When all conditions met

Lactose present

Glucose scarce

CAMP levels high

Constituive Expression

Regulatory

Trp R

Lac I

Trp A

Trp B

Trp C

Trp D

Trp E

Lac A

Beta Galactosidase acetylase

Lac Y

Permease

Lac Z

Beta Galactosidase

Switch: Operator

OFF

Negative
Repressor

Basal level

ON

Positive
Activator

Increased RNA transcription

Formylmethionine first amino acid in protein

Trp operon

Lac operon

STRUCTURE MAKES FUNCTION

Energy

Photosynthesis
Alt. mechanisms for Carbon Fixation

CAM plants

open their stomata at night

C4 plants

then exported to bundle-sheath cells

Incorporate CO2 in mesophyll cells

C3 plants

Close stomata conserving water

Photorespiration

O2 subs for CO2 in rubisco sites

Calvin Cycle

One molecule of G3P exits per three CO2

Uses ATP and NADPH to reduce CO2 and sugar

Light reactions

Electron flow

Cyclic

only one PS is used, produces only ATP

Linear

Uses both PS's and produces, NADPH, ATP, AND O2

Photosytems

Both contain a reaction-center complex and a light harvesting complex

PS II

P680 molecules

PS I

P700 molecules

Photon

Pigment absorbs

Wavelengths

Visible light wavelengths drive photosynthesis

Photosynthesis makes food

Photosynthesis is a redox

CO2 is reduced

H2O is oxidized

6CO2 + 12H2O + Light Energy --> C6H12O6 + 6O2 + 6H2O

Choloroplasts

Stroma

Uses NADPH for reducing power

Uses ATP for energy

CO2--> sugar

Calvin cycle

grana

Thylakoid

Produce ATP and form NADHP

release O2

split water

Where light reactions occur

autotrophic

Makes its own food

Cellular Respiration
Anaerobic respiration

Fermentation

Lactic acid fermentation

pyruvate is reduced by NADH to form lactate

Alcohol fermentation

Pyruvate is converted to ethanol

Oxidative phosphorylation

Chemiosmosis

26 or 28 ATP produced

H+ concentration gradient on the outside of the inner membrane couples the redox reactions of the ETC

makes ATP from ADP and inorganic phosphate

ATP Synthase

Electron carriers

Cytochromes

FADH2

NAD+

Electron Transport Chain

Occurs in the inner mitochondrial membrane

Total: 2 e-, 2H+, 1/2 O2

The electrons travel from complex I and II to complex Q, then to Complex 3 followed by complex 4.

When a complex receives en electron, it is reduced, then oxidized when it is passed onto the next complex

Citric acid cycle

Total yield

One glucose--> 6 NADH, 2FADH2, 2 ATP

Step 4

Another CO2 is lost, NAD+ becomes NADH. a-ketoglutrate becomes Succinyl CoA

Isocitrate is oxidized, reducing NAD+ to NADH and losing a CO2 molecule. Isocitrate becomes a-ketoglutarate

Acetyl CoA becomes citrate

Two reduced carbons enter the cycle with Acetyl CoA, and two oxidized carbons leave in the form of CO2

Pyruvate oxidation

Acetyl CoA is formed via coenzyme A

Step 2

Remaining fragment is oxidized forming acetate and releasing electrons forming NADH.

Methionine first amino acid placed in protein

Pyruvate dehydrogenase complex removes the CO2 from the pyruvate.

Occurs in the mitochondrial matrix

Glycolysis

Occurs in the cytosol

Net

Glucose --> 2 Pyruvate + 2 H2O 2 ATP, 2 NADH, + 2 H+

Energy Payoff Phase

4 ATP formed, 2 NADH, 2 H+, 2 pyruvate, 2 H2O

Energy Investment Phase

Step 5

Two three carbon sugars are left (Glyceraldehyde 3-phosphate and Dihydroxyacetone Phosphate)

G3P and DHAP which convert between each other

Step 3

Phosphofructokinase transfers a phosphate from ATP to opposite end of the sugar (this is the second ATP)

Step 1

Hexokinase transfers phosphate from ATP to Glucose

Glycolysis occurs whether or not O2 is present

Catabolic Pathway, redox

Powered by redox reactions

Substrate-level phosphorylation

Phosphate for ADP comes from substrate rather that an inorganic phosphate

NADH reduced state

NAD+ oxidized state

RIG (oxidizing agent)

OIL (Reducing agent)

Metabolism
Cooperativity

affects other binding sites

amplifies enzyme response

substrate binds to active sites

allosteric activation

Enzyme Regulation

Enzymes and substrates are compartmentalized within the cell

Allosteric Regulation

Inhibitors

Activators

stabilize subunits

Oscillating subunits

Not bound on active site

Enzymes

How they work

Non competitive inhibitors

Bind somewhere other than active site

Competitive inhibitors

Bind to active site

Cofactors

Non protein helpers

Optimal conditions

Temperature

pH

Induced fit via malleable equilibrium

Enzyme -substrate complex

Converts substrate to products

They lower Ea

Catalyst

Activation Energy

Transition state

Has enough energy to break bonds

Energy required to get to the "top of the hill"

Coupling

Phosphorylation

Coupling occurs by endergonic and exergonic reactions fueling each other.

Phosphorylated intermediate: key to coupling

Work

Mechanical Work

Transport Work

Chemical Work

Energy Transformation

Thermodynamics

Large amount of energy transferred is lost as heat

Entropy and Enthalpy

2nd Law: Energy transfer increases entropy of Universe

1st Law: Principle of Conservation of Energy

Surrounding vs. system

Chemical Energy

Heat: transfer of thermal energy

Thermal Energy

Kinetic Energy

Metabolic Pathways

Anabolic Pathway

Catabolic Pathway

Free Energy

Images

Subtopic

ΔG

ΔG = ΔG final - ΔG initial

ΔG< 0 , spontaneous, exergonic

ΔG> 0, non-spontaneous, endergonic

ΔG = ΔH -TΔS

When temp. and pressure are uniform

Universe = System + Surroundings

Equilibrium

Equilibrium= Dead System

System never moves away from Equilibrium Spontaneously

Free energy decreases closer to equilibrium & Vice Versa.

Max stability = Equilibrium

ΔG = 0 , Equilibrium

Prokaryotes

Genes

Expression
Regulation
Protein Processing
Translation
Transport to cytoplasm
RNA Processing
transcription
Level

Chemistry of life

Eukarytotes

Macromolecules

Carbohydrates
Polysaccharide

Structural

Cellulose

Storage

Glycogen

Starch

Disaccharide

Sucrose

Lactose

Monosaccharide

Glucose

Fructose

Lipids
Steroids

Testosterone (hormones)

Cholesterol

Trigycerol

Unsaturated fats

Important Energy source (oils)

Saturated fats

Energy source (butter)

Phospholipids

Phospholipid bilayer

Nucleus Acids
Nucleotide
RNA
DNA