Unit 4: Genetics

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Unit 4: Genetics

Biotechnology Techniques

Gel Electrophoresis

Visualizing Gel

Ethidium Bromide

Loading Dye

Gel Medium

Polyacrylamide

Agarose

DNA Amplification

Taq Polymerase

Polymerase Chain Reaction

DNA Strand Synthesis

Primer Annealing

DNA Strand Denaturation

Recombinant DNA

Restriction Enzyme Digestion

DNA Isolation

Gene Regulation

Post-Translational

Post-Transcriptional

Pre-Transcriptional

Synthesized Protain

Translational

Transcriptional

Bacteria

trp operon

lac operon

Terms

Activator

Repressor

Promoter

Operator

Operon

Housekeeping Genes

Mutations

Disorders

Crohn's Disease

Cystic Fibrosis

Photo Repair

Chromosomal Mutation

Reciprocal Translocation

Single Gene Mutation

Frameshift

Deletion

Insertion

Substitution

Mis-Sense

Non-Sense

Silent

Causes

Chemical

Physical

Spontaneous

Genetic Code

Universal

Continuous

Redundant

Molecular Chaperones

Stop Codon

UAA

UAG

UGA

Subtopic

Active Ribosome

Start Codon

Acceptor Stem

Anticodon Loop

Post-Transcription

Removal of Introns

Poly-A Tail

5' cap

Terminator Sequence

Sense Strand

Antisense Strand

Promoter Region

RNA Polymerase

Central Dogma of Gene Expression

RNA

tRNA

rRNA

mRNA

Translation

Transcription

DNA Replication

Phases

Termination

Telomerase

Telomeres

Elongation

DNA Ligase

Lagging Strand

Leading Strand

DNA Polymerase III

RNA Primase

RNA Primers

Initiation

Replication Forks

Topoisomerase II

Meselson and Stahl

15N

14N

Models

Dispersive

Conservative

Historical Contributions

Watson & Crick

Semiconservative

Franklin

0.34nm/3.4nm

Sugar Phosphate Backbone

Pauling

Helix Shape

Chargaff

Chargaff's Rule

C:G

A:T

Hershey & Chase

S35

P32

Griffith

Transformation

Non-Virulent R Strain

Virulent S-Strain

Miescher

Nuclein

Nuclei

Molecular Genetics

Gene

Genome

Organization

Chromosomes

Chromatin

Nucleosome

Eukaryotes

Nucleus

Prokaryotes

Plasmid

DNA

Anti-Parallel Strands

Complementary Strands

Double Helix